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Image Search Results
Journal: Molecular Therapy. Nucleic Acids
Article Title: A novel function and mechanism of ischemia-induced retinal astrocyte-derived exosomes for RGC apoptosis of ischemic retinopathy
doi: 10.1016/j.omtn.2024.102209
Figure Lengend Snippet: miR-329-5p protected the retina from ischemic-reperfusion injury by targeting the MAPK pathway (A and B) Venn diagrams showing the intersection of predicted target genes of hsa-miR-329-5p and mmu-miR-329-5p, and MAPK8 (Jnk1) is a potential gene targeted by both miRNAs. (C) Real-time qPCR analysis of MAPK8 mRNA expression after intravitreal treatment of PBS, scrambled miRNA (miR-NC), and miR-329-5p (n = 5). (D and E) WB and quantification of protein expressions of MAPK8- related pathway on retinal tissues after intravitreal treatments of PBS, miR-NC, and miR-329-5p (n = 5). (F) IHC of MAPK8 expression on retinal tissues after various intravitreal treatments (n = 5 animals; scale bar, 100 μm). (G) Dual-luciferase reporter gene plasmid design and the predicted target sites. (H) The dual luciferase reporter gene assay showed that miR-329-5p significantly inhibited the luciferase activity of the MAPK8 -WT1 3′UTR reporter gene in 293T cells. Data was presented as mean ± SD, n = 3. Student’s t test was used for statistical analysis. Data were statistically analyzed by one-way ANOVA followed by Tukey’s post hoc analysis. Statistical significance at ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001. ns, not statistically significant.
Article Snippet:
Techniques: Expressing, Luciferase, Plasmid Preparation, Reporter Gene Assay, Activity Assay
Journal: Molecular Therapy. Nucleic Acids
Article Title: A novel function and mechanism of ischemia-induced retinal astrocyte-derived exosomes for RGC apoptosis of ischemic retinopathy
doi: 10.1016/j.omtn.2024.102209
Figure Lengend Snippet: Antibodies used in this study
Article Snippet:
Techniques:
Journal: Molecular Therapy. Nucleic Acids
Article Title: A novel function and mechanism of ischemia-induced retinal astrocyte-derived exosomes for RGC apoptosis of ischemic retinopathy
doi: 10.1016/j.omtn.2024.102209
Figure Lengend Snippet: Primer sequences used in real-time qPCR test
Article Snippet:
Techniques: Sequencing